Fabrication of micropillar substrates using replicas of alpha-particle irradiated and chemically etched PADC films.
نویسندگان
چکیده
We proposed a simple method to fabricate micropillar substrates. Polyallyldiglycol carbonate (PADC) films were irradiated by alpha particles and then chemically etched to form a cast with micron-scale spherical pores. A polydimethylsiloxane (PDMS) replica of this PADC film gave a micropillar substrate with micron-scale spherical pillars. HeLa cells cultured on such a micropillar substrate had significantly larger percentage of cells entering S-phase, attached cell numbers and cell spreading areas.
منابع مشابه
Proliferation of epithelial cells on PDMS substrates with micropillars fabricated with different curvature characteristics.
The present work studied the proliferation of epithelial cells when they were cultivated on substrates with micropillars fabricated with the same height but with different curvature characteristics. A special micro-fabrication method was employed to produce these micropillar substrates. Polyallyldiglycol carbonate (PADC) films were first irradiated by alpha particles and then chemically etched ...
متن کاملFabrication of pseudo three-dimensional PADC cell culture substrates for dosimetric studies
Pseudo three-dimensional (3D) polyallyldiglycol carbonate (PADC) cell culture substrates were fabricated by creating micrometer-size pores on thin PADC films by alpha-particle irradiation and subsequent chemical etching. Our results showed comparatively fewer 53BP1 foci in alpha-particle irradiated as well as bystander HeLa cells cultured on pseudo 3D PADC substrates than in those cultured on t...
متن کاملMicro-collimators fabricated by chemical etching of thin polyallyldiglycol carbonate polymer films exposed to oxygen ions
One approach for a particle radiobiological experiments is to use solid-state nuclear track detectors (SSNTDs) as support substrates to record the a particle hit positions on the targets. To facilitate accurate characterization of the hit positions as well as the incident a particle energies, micro-collimators are required in these experiments to restrict the incidentaparticles to thosewith sma...
متن کاملMicro-collimator fabricated by alpha-particle irradiation of polyallyldiglycol carbonate polymer film and subsequent chemical etching
In the present paper, we propose a method to fabricate a ‘‘micro-collimator’’ with a thickness of 15mm. A commercially available PADC film with a thickness of 100mm was first chemically etched with NaOH/ethanol solution to obtain a thin PADC film. This thin PADC film was then irradiated by 5MeV a particles through a ‘‘macro-collimator’’ with a thickness of 5mm and a hole diameter of 1mm. This P...
متن کاملDosimetric study of radioadaptive response of zebrafish embryos using PADC-film substrates
We found the alpha-particle priming dose which could effectively induce in vivo radioadaptive response in the embryos of the zebrafish, Danio rerio, to be 4.8e48 mGy, through the apoptotic signals scored in the embryos at 24 h post fertilization. Specially fabricated thin polyallyldiglycol carbonate (PADC) films were used as support substrates for the dechorionated embryos. During irradiation, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine
دوره 70 7 شماره
صفحات -
تاریخ انتشار 2012